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The current version of the Tweetbot beta does not allow for any new users or for current users to add any new accounts.
Tweetbot for Mac is finally available! However it may not be quite as you had hoped. Developing for the Mac is no easy task, especially a full-featured Twitter client. However, we've gotten to a point where while not complete, it is useable. We've decided to release it as a public alpha to a) motivate us to finish faster, and b) get feedback to help us build the best Mac incarnation of Tweetbot we can make. You can love it or hate it, but rest assured it will only get much much better from here.