v12.6
- There is a brand new interactive primer design interface that lets you quickly view the properties of any primer, see instantly where it binds on any open sequence window and even nudge the primer on a sequence to find the optimal primer characterstics. You can edit the primer to introduce mismatches and see the impact of the mismatches on translations crossing the binding site and even add tails containing restriction site. Finally, you can run Primer3 from the Quicktest dialog to find a matching primer suitable for PCR and copy a DNA sequence containing the predicted PCR product, complete with any mismatches or tails introduced by the primers. The new functionality is invoked from the Analyze Primers Quicktest Primer menu item. For more details, please read the new "Primer Design Tutorial.pdf" document in the /MacVector 12.6/Documentation/ folder.Feature Import
- You can now import features from GFF, GFF3, GTF and BED files. To use this, open the parental sequence and choose File Import Features. Perfectly duplicated features will be discarded, so you can use this to repeatedly import features from an external source where the features in the source file may be updated from time to time without having to start over from scratch. This feature allows you to import annotation directly from many enome Browsers such as the UCSC Genome Browser. Phrap de novo Assembly of NGS data The add-on Assembler module has been enhanced to allow phrap assemblies of file-based (i.e. FastQ) formatted sequences. Please see the Assembler Release Notes for more details.Miscellaneous Enhancements
- The Map graphics layout and drawing performance have been speeded up. In particular, MacVector can now display all of the features from a typical bacterial chromosome almost instantaneously and lets you zoom in and out from interesting regions extremely quickly. You can now edit the LOCUS name in DNA sequences. If you option-click on a Read sequence in the Align to Reference Editor, the corresponding trace chromatogram will slide into view in the lower multiple-trace pane. This is particularly useful if you have hundreds of aligned clonal variants and want to quickly inspect the chromatogram to validate mutations. Sorting an Align to Reference assembly now ensures assembled Reads always float to the top. All Tm calculations now use the updated thermodynamic nearest neighbor parameters of Santa Lucia (1998) and also take into account divalent cation concentration and dNTP concentrations as described by von Ahsen et al (2001). You can now save all open sequences into a single GenBank file
