FlowJo versions

Scientific analysis program designed for flow cytometric data.
May 16, 2023
Dec 3, 2021
Apr 26, 2018
Nov 30, 2023
6.3
Review

What's new

v6.3
- Significant performance improvement noticeable when compiling large layouts or tables-particularly when hundreds or thousands of samples are involved!
- Fix text issues in dialogs on some computers running OS9.2.2.
- Summit 4.x files with incorrect data location specification keywords are now read in properly.
- The compensation definition window can now be resized to allow for long parameter names.
- Fixed: Memory was slowly being used up by doing large numbers of text boxes in the Layout Editor
- Fixed: Tables with negative numbers in the statistics will now show means & standard deviations.
- A number of optimizations and bug fixes were done to prevent crashes that occur during large computational demands; stability should be much better.
- Site licensees are validated more efficientlyPreferences:
- There is a new preference to define the default sort order for samples in the workspace Workspaces:
- Workspaces are now read in and saved much more efficiently. Note: the first time you read in an older workspace, it may be much slower; as soon as you save in the new format, it will be considerably faster!
- Workspace sort criteria are now "persistent": if you add new files (or for templates), the files will be automatically sorted according to the last selected criteria. In addition, when you define the sort criteria, you can make them the default for all new workspaces.
- Workspace sort criteria can be individually set to be increasing or decreasing values.
- Unreferenced samples are now removed from workspaces (making templates much smaller).
- XML format has been modified (more similar to PC version). This format is still being updated and is not guaranteed to be consistent with future revisions of FlowJo.Tables:
- Tables with keyword values that have or display properly
- You can copy a series of custom column heading names (from Excel or Word, separated by returns) into any column heading name - the entire series is copied to the subsequent table columns (hit return or tab after pasting). In this way, you can edit the column names in a different program and then copy them in all at once.
- The mean and std. dev. are filled in only for valid calculations (e.g., they are left blank for text columns)Bug fixes:
- Fix: Preferences correctly reads layout color palette for overlay colors
- Fix: Bug that could cause rare occasional crashes when reading files
- Fix: When choosing to sort the workspace, the current sort criteria are displayed
- Templates that refer to compensated parameters now add all gates & statistics
- Renaming a gate will no longer cause a Layout (referring to that gate) to crash
- Opening graph windows no longer occasionally reset the Layout iteration value
- Cycling graphs (next/previous) no longer cause a rare crash that happens when gate frequencies were shown
- Modifying a series of gates that are all group-synchronized no longer causes a rare crash
- $SPILLOVER keyword values are properly supported
- Allow dragging gates from one workspace to another if the destination sample is selected
- Crash no longer occurs if changing a graph while an outlier plot is computed
- Selecting a graph from a sample not in the current group does not make that sample temporarily appear in the workspace window
- Double clicking multiple samples in the control definition list for an overlay graphic now sets all selected samples as controls
- Grid lines are displayed in histogram overlays
- Histogram overlays will not occasionally display parameter names for the Y axis label
- Boolean gate specifications are no longer case-sensitive
- The Layout batch Preview will not crash if the display is too large to show on-screen
- The line color for boxes drawn around text is correct in batched layouts
- Summit data files with badly-formatted $SPILL keyword (Summit version 4.1) are now read in (an error message is shown) but no compensation parameters are added. These files can be compensated the usual way in FlowJo, using appropriate compensation controls.
- FlowJo does not crash if you try to delete the only layer listed in the Legend Item for a Layout Graphic.
- Batch layout generation: if Iterate by Nth (n > 1), the resulting layout's discriminator is properly set.

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